Endo-β-1相关论文
Gene cloning,heterologous expression and characterization of a Coprinopsis cinerea endo-β-1,3(4)-glu
A gene coding endo-β-1,3(4)-glucanase(ENG16A)was cloned from Coprinopsis cinerea and heterologously expressed in Pichia......
P42Thermophilic β-1,4-EndoglucanaseCrystal Structure Unveils the Thermostability and Catalytic Mecha
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,Hydrolysis of filter-paper cellulose to glucose by two recombinant endogenous glycosyl hydrolases o
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Two novel endo-β-1,4-glucanases, EG45 and EG27, were isolated from the gastric juice of mollusca, Ampullaria crossean, ......
,The N-terminal cellulose-binding domain of EGXA increases thermal stability of xylanase and changes
A full-length EGXA enzyme from a mollusk, Ampullaria crossean, was cloned into pFastBac vector and then heterogeneously ......
,Purification and characterization of a novel endo-β-1,4-glucanase,AfEG22,from the giant snail,Achat
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,Purification and characterization of a salt-tolerant cellulase from the mangrove oyster, Crassostre
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采用PTDS方法人工合成米曲霉葡聚糖酶基因(AoEGLAI),通过电击将其转化到毕赤酵母中表达,并采用DNS法对重组酶的酶学性质进行分析.......
采用不同饱和度的硫酸铵溶液对内切β-1,3-葡聚糖酶粗酶液进行分段盐析,以确定最佳盐析条件,将盐析处理后的酶液经透析浓缩、DEAE-......
通过RT-PCR克隆、鉴定了黑曲霉中编码1种葡聚糖酶基因eglA6,所编码的重组酶蛋白命名为AneglA6。通过密码子优化提高了AneglA6在巴......
利用RT-PCR技术，提取黑曲霉（Aspergillus niger）L3的总RNA进行反转录，并通过PCR扩增得到去除天然信号肽的β-1，4-葡聚糖酶基因eg Ⅰ，将其......
利用PCR技术从冰岛硫化叶菌（Sulfolobus islandicus）REY15A中分别扩增得到带有和不带有信号肽编码序列的β-1,4-内切葡聚糖酶基因（S.i......
提取了台湾家白蚁总RNA并反转录获得eDNA，PCR扩增出白蚁内切葡聚糖酶的基因，并将目的基因分别克隆到大肠杆菌和酿酒酵母载体中，构建了......
采用PTDS方法人工合成米曲霉葡聚糖酶基因（AoEGLAI）,通过电击将其转化到毕赤酵母中表达,并采用DNS法对重组酶的酶学性质进行分析。结......
根据寡糖与显色剂反应后的呈色物质在540nm处吸光值强度，建立了一种用于分析水解液中寡糖浓度的定量薄层层析法。应用阴离子交换层......
在不同的培养条件（如接种量、初始pH、温度、转速、不同碳氮源等）下，对土曲霉M11菌丝球的形态与产酶量的关系进行了深入研究，可以观察......
以成熟的莱阳茌梨果实为试材,应用同源克隆和RACE方法从茌梨果肉中克隆到内切-β-1,4-葡聚糖酶基因的一个同源基因cDNA的全长序列,......
哈茨木霉的深层培养菌体形态与β-1,3-葡聚糖内切酶酶活之间有密切关系。通过单因素试验优化了控制哈茨木霉菌球形态的培养条件为：......
以番茄为例，对β-1，4-甘露聚糖内切酶EC3．2．1．78在种子发芽、花药花粉发育和果实成熟等过程中所充当的角色加以综述，进而说明该酶的功能......
经丙酮沉淀、Ultrogel AcA54凝胶过滤、Q-Sepharose离子交换柱层析和聚丙烯酰胺凝胶制备电泳,发现桑粒肩天牛幼虫肠液中具有所有水......
随着人们对农产品品质和安全性要求的提高,高效生物防治资源的筛选显得尤其重要。本研究通过平板对峙实验、分子鉴定、刚果红染色......
从土壤中分离到一株产纤维素酶的巨大芽孢杆菌AP25，经羧甲基纤维素平板检测，该菌可产生葡聚糖内切酶。根据GenBank登录的β-1，4内切葡......
首先通过引物设计扩增嗜热内切-β-1，4-葡聚糖酶基因，然后采用NotI和XhoI双酶切目的基因与载体pPICZct—A，通过T4连接酶连接后转化DH5......
Cloning and Expression of Extremely Heat-Resistant Endo-β-1,4-Glucanase Gene from Thermotoga maritim
Gene encoding endo-β-1,4-glucanase(TM1525)is derived from Thermotoga maritima(T.maritima),which has an open reading fra......
